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DuPont de Nemours
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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Elucidating the mechanism of triphenyl phosphate interference in bone metabolism via network toxicology and molecular docking methodologies
doi: 10.3389/fendo.2025.1606877
Figure Lengend Snippet: Effects of TPHP on osteoblast function. (A, B) TPhP inhibits cell migration (Wound healing assay). (C) TPhP reduces cell proliferation (CCK-8 assay). (D, E) TPhP suppresses migration and invasion (Transwell assay). (F) Western blot showing TPhP effects on MAPK and EMT proteins. (G, H) MEK/ERK activator (C16-PAF) rescues TPhP-inhibited migration/invasion (G) and proliferation (H) , while an inhibitor (MEK-IN-6) does not. (I) Western blot confirming MAPK/ERK modulation in rescue experiments. (J) qPCR showing TPhP downregulates key target genes.(*P < 0.05, **P < 0.01, ***P < 0.001).
Article Snippet: For pathway validation experiments, cells were treated with TPhP (40 μM) alone or in combination with the
Techniques: Migration, Wound Healing Assay, CCK-8 Assay, Transwell Assay, Western Blot